How to use the pre-rolled tube in the laboratory

Update:03-11-2021
Summary:

Prepare a sterile work area Before beginning any disinf […]

Prepare a sterile work area
Before beginning any disinfection procedures in your work area, wash your hands thoroughly with disinfectant soap and warm water.
Be sure to re-wash your hands any time you suspect that you have contamination of your experimental operation.
Clear all materials on the benches of the laboratory stuffed with your work area. Remove the pre-dipped disinfectant wipe canister and wipe the entire area. Allow the disinfectant to evaporate-don't wipe it dry!
Use disinfectants such as alcohol (isopropanol or 70% ethanol) or phenolic compounds (o-phenyl).
In order to prevent the atomization, production or the diffusion of fine mist containing bacterial cells, microbial contaminants, and to avoid the disinfection of the dispensing from the bottle washing.
Dry microorganisms are one of the most effective ways to purify the surface. Even if someone has recently wiped out the test bench and benchtop with disinfectant, always wipe the bench to start your test time. After the disinfectant is completely dry, use an igniter to light the Bunsen burner. Adjust the flame so that you can see a blue cone in the flame. Now, with the updraft generated by the flame, or air convection, the warm air rises from the flame (Figure 1). As the heat rises, microorganisms and dust particles are forced upwards, away from the work area in front of them. Work slowly, carefully, and deliberately all the time in this area created by the Bunsen burner, called the sterile field. Maintain the Bunsen burner throughout the process.
The blue cone tip flame is the hottest part.
Be careful not to disturb the fast-moving updraft, which greatly changes the current around the AIŕ test bench. Creating the updraft of the Bunsen burner minimizes the possibility of microbes and dust fall from opening bottles, tubes or bottles on the bench or in the work area.
Arrange all supplies needed for the procedure on the laboratory bench near the sterile area. Make sure all materials are properly labeled.
Supplies may include serum micropipettors and pipettes, sterile culture tubes, sterile bottles, media bottles containing broth, sterile centrifuge tubes, pipette tips, tube racks, bacterial cell culture and phage stocks.
The liquid medium should be sterilized at 121°C and the liquid set for at least 15 minutes in a reactor. Larger media volumes (> 1L) require longer reactor times. Laboratory equipment should be sterilized for at least 30 minutes, with gravity (dry) set in an autoclave at 121°C.
In general, the sterile solution can be stored at 4°C for up to 5 months. Please note that the storage time is significantly reduced for solutions containing antibiotics, such as unstable components-always check the manufacturer's recommendations.
Use a serum dropper to transfer liquid
Serological pipettes come in many sizes and options: disposable plastic or glass, or reusable, inserted or unplugged. These calibration deliveries range from 0.1 ml to 25 ml.
Common sizes for serological pipettes are 5ml, 10ml and 25ml, and should be 0.1ml or more (panel picture 2) to use sterile liquid transfer. There are also some larger serological pipettes that can provide volumes up to 100 ml, however, the focus of this protocol is on the more common, smaller size pipettes.
Pre-sterilized cotton wool insert pipettes require microbiology and tissue culture experiments. The plug should not be removed from the p-pipette, it is intended to act as an obstacle to an overflowing pipette.

Different application requirements for plastic and glass serological pipettes. Organic solvents required for glass. It can be used when performing BSL-1 experiments on the top of the experimental platform. BSL-2 can use the only plastic when the organisms that cannot use the Bunsen burner work in the biological safety cabinet. It also suggests that plastic melting agar involves transfer applications.
There are two types of serological pipettes: training class ("contains") or TD ("delivery"). All volumes provided by the training class pipette, including the tip, must be "blown out" or rinsed to obtain the specified volume. The TD pipette calibration should not be delivered with the tip left a little bit. Be sure to check the top of the label near the body to determine which type of straw it is (Figure 3). The most commonly used is the TD pipette, which is the double circle with KED at the top.
Take a sterile plastic serological pipette (also called a volumetric pipette) and carefully remove the cotton tampon paper sleeve at the end of the peeling skin like a banana-do not remove the entire sleeve, protect the tip of the pipette, which will be transferred Liquid contact. Touch only the top of the straw (the graduation quotation marks above) by hand.
Never enter a sterile solution, use a pipette, even if precautions have been taken to keep it sterile.
Glass serological pipettes are usually stored in metal cans (Panel B in Figure 2). Loosen the top of the can, and then carefully remove the cap, flame lid, and can's opening lid. Place the cap under, on its side, to sterilize the bench. Remove the pipette horizontally from the jar and shake it gently so that the top TES of one or two pipettes protrudes about an inch, which can be easily grasped. Put down the jar on its side and remove a pipette, but be careful not to touch the other pipettes in the container. With your hands, do not touch the tip of the straw and avoid contact with the tip of other non-sterile surfaces.
Affix the tip of a bulb, pump, gun or serological pipette, such as a pipette aid. Paper sleeve from plastic straws. Hold the straw for aid in your right hand.
Although it is best to avoid use, if you must take the cover off, place it on a surface that faces the disinfection. With a hat facing upward, there is a greater chance that contamination from objects or hand movements will cause microbes and dust particles to drop to the airflow on the inner surface of the cap.
The purpose of burning is not to sterilize, but to warm the opening Øf of the bottle, creating convection of air leaving the curtain (ie updraft). Warm, the air rises, helping to prevent dust particles and other contaminants from entering the bottle.
Keep sterile containers for as little time as possible. It is important to keep the microorganisms in the air entering the lowest point of the whole process.

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