The operation and use of glass instruments commonly used in pipettes

1. Classification:
Single reticle pipette (also known as belly pipette), indexing pipette (incomplete flow type, complete flow type, blow-out type)
2. A single reticle pipette is used to accurately pipette a certain volume of solution
The marking part of the single-marked pipette has a small diameter and high accuracy; the scale part of the graduated pipette has a large diameter and a slightly poor accuracy. Therefore, when taking an integer volume of solution, the corresponding size is often used. Single reticle pipette instead of graduated pipette.

3. Operation:
Pipetting: For experiments that require relatively high accuracy, blow off the remaining water on the tip, then use filter paper to wipe off the water inside and outside the tip of the tube, and then rinse with the liquid to be taken three times to ensure that the concentration of the pipetting operation solution remains unchanged. . Be careful not to reflux the solution to avoid dilution and contamination of the solution.
When pipetting the solution to be suctioned, insert the tip of the tube 1-2cm below the liquid surface (too deep: too much solution adheres to the outer wall of the tube; too shallow: suction empty after the liquid level drops)
Reading: The line of sight is at the same level as the lowest point of the meniscus of the solution.
Release: The tip of the tube touches the inner wall of the vessel, so that the container is tilted and the tube is upright, allowing the solution to remain free along the wall; before the pipette is removed from the receiving container, wait for 3s to ensure that the liquid flows out completely.