Tips, as consumables used with pipettes, generally can […]
Tips, as consumables used with pipettes, generally can be divided into: ①. Standard tips, ②. Filter tips, ③. Low adsorption tips, ④. No heat source, etc.
1. The standard tip is a widely used tip. Almost all pipetting operations can be used with ordinary tips. This is the most affordable type of tips.
1. The filter tip is a consumable designed to avoid cross-contamination. It is often used in experiments such as molecular biology, cytology, and virology.
2. For experiments with high sensitivity requirements, or precious samples or reagents that are easy to remain, you can choose a low-adsorption tip to increase the recovery rate. The surface of the low-adsorption tip has undergone a hydrophobic treatment, which can reduce the low surface tension liquid leaving more residues in the tip.
How to choose a good tip?
The tip that can be mounted on the pipette can indeed form a pipetting system with the pipette to realize the pipetting function, but is this reliable?
1. You may wish to perform a performance test after matching the pipette with the tip. After rinsing the tip, perform several repeated sample addition operations, weigh the sample addition amount each time, and record the reading.
2. Calculate the accuracy of the pipetting operation after converting it into volume according to the density of the test liquid
3. What we have to choose is to have a good suction head. If the pipette and the pipette tip are not well matched, it means that the tightness of the pipette and the pipette cannot be guaranteed, so that the results of each operation cannot be reproduced.
So what are the minimum points for a good tip?
A good tip depends on concentricity, taper, and the most important point is adsorption;
1. Taper: If it is better, the match with the gun is very good.
2. Concentricity: The concentricity is whether the circle between the tip of the tip and the link between the tip and the pipette is the same center. If it is not the same center, it means that the concentricity is not good;
3. Adsorption: Adsorption is related to the material of the tip. If the material of the tip is not good, it will affect the accuracy of pipetting, resulting in a large amount of liquid retention or simply called wall hanging, resulting in pipetting errors.