What are the functions and precautions of centrifuge tubes?

1. Type:
A large number of centrifuge tubes (500mL, 250mL) ordinary centrifuge tubes (50mL, 15mL) microcentrifuge tubes (2mL, 1.5mL, 0.65mL, 0.2mL)
The centrifuge tube follows the shape of the bottom:
Conical centrifuge tube, the bottom is tapered, it is the most used centrifuge tube type, flat bottom centrifuge tube, round bottom centrifuge tube
The centrifuge tube is closed according to the lid:
Gland centrifuge tubes, centrifuge tubes sealed by pressing, are common in micro centrifuge tubes, screw cap centrifuge tubes, and can be divided into flat caps (the top of the cap is flat) and plug caps (the top of the cap has a plug shape)

Centrifuge tubes are divided into materials:
1. Glass centrifuge tube: The centrifugal force should not be too large when using glass tubes. Rubber pads are needed to prevent the tube from breaking. High-speed centrifuges generally do not use glass tubes. The cap of the centrifuge tube is not well sealed, and the liquid cannot be filled up (for high-speed centrifuges and angle rotors are used) to prevent overflow and loss of balance. The consequence of spillage is to contaminate the rotor and the centrifugal cavity, and affect the normal operation of the sensor. During ultracentrifugation, the liquid must be filled with the centrifuge tube, because ultra-separation requires a high vacuum, and only by filling it can the centrifuge tube be prevented from being deformed.
2. Steel centrifuge tube: Steel centrifuge tube has high strength, no deformation, heat resistance, frost resistance, and chemical corrosion resistance. Its application is also quite wide, but it should also avoid contact with strong corrosive chemicals when using it, such as Strong acid, strong alkali, etc. Try to avoid the corrosion of these chemicals.
3. Plastic centrifuge tube: The advantage of plastic centrifuge tube is that it is transparent or translucent, and its hardness is small, so the sample can be taken out by puncture method. The disadvantage is that it is easily deformed, has poor resistance to organic solvents, and has a short service life. Plastic centrifuge tubes have caps. Its function is to prevent sample leakage, especially when it is used for radioactive or highly corrosive samples. It is very important to prevent sample leakage; the tube cap also has a function to prevent sample volatilization. And support the centrifuge tube to prevent deformation of the centrifuge tube.
2. the role of the tube cover
Plastic centrifuge tubes are equipped with tube caps, and the tube caps must be tightly closed to prevent liquid from leaking out.
The role of the cap:
1. When used for radioactive or highly corrosive samples, prevent the samples from leaking.
2. Prevent the sample from volatilizing.
3. Support the centrifuge tube to prevent the centrifuge tube from deforming.