What are the precautions for vacuum blood collection

1. Selection and injection sequence of vacuum vessels
According to the test items, select the corresponding test tube. The sequence of blood injection was culture bottle, common test tube, test tube with solid anticoagulant and test tube with liquid anticoagulant. The purpose of this order is to minimize the analysis error caused by sample collection. Blood distribution sequence: ① sequence of using glass test tube: blood culture test tube, serum tube without anticoagulant, sodium citrate anticoagulant test tube, other anticoagulant test tubes. 2. Use plastic test tube sequence: blood culture tube (yellow), citrate anticoagulation test tube (blue), serum tube added or not added with blood coagulating activator or gel, gel or ungelated heparin tube (green), EDTA anticoagulant tube (purple), and blood sugar determination inhibitor test tube (grey).

2. Blood sampling position and posture
According to the method recommended by the World Health Organization, the blood of the thumb or the internal and external sides of the heel can be taken, and the first choice is the vein at the head and neck or the vein at the anterior fontanelle. In adults, the median elbow vein, dorsum of hand and wrist joint without congestion and edema were selected, and the veins of individual patients were on the back of elbow joint. Patients in outpatient department take sitting position and patients in ward take lying position. When taking blood, ask the patient to relax, keep the environment warm, prevent vein contracture, the binding time should not be too long, and it is forbidden to pat the arm, otherwise it may cause local blood concentration or activate the coagulation system. As far as possible, choose the large and easy to fix blood vessels for puncture, to ensure that the point of view. The angle of needle insertion is generally 20-30 °. After the return of blood is seen, the needle enters in parallel and forward slightly, and then a vacuum tube is put on. The blood pressure of some patients is low. After the puncture, there is no return of blood, but after the negative pressure tube is put on, the blood flows out smoothly.
3. Strictly check the validity of blood collection
It must be used within the validity period, and shall not be used when there are foreign matters or deposits in the blood vessels.
4. Paste barcode correctly
Print the barcode according to the doctor's instructions, paste it on the front after checking, and the barcode can't cover the scale of blood collection vessel.
5. Timely inspection
Blood samples should be sent for examination within 2 hours after collection to minimize the influence factors. When sending for inspection, avoid strong light, wind, rain, freezing, high temperature, shaking and hemolysis.
6. Storage temperature
If the storage temperature is 0 ℃ or lower than 0 ℃, it may cause the rupture of the blood vessels.
7. Protective latex sleeve
The latex sleeve at the needle end of the prick plug can prevent the blood collection tube from bleeding and polluting the surrounding area after it is pulled out, which plays the role of sealing the blood collection and preventing environmental pollution. The latex sleeve shall not be removed. When collecting blood samples from multiple tubes, the rubber of the needle may be damaged. If there is any damage, causing blood overflow, it should be absorbed first and then disinfected.