Precautions for Pipette Use

Update:24-07-2020
Summary:

1. Pipettes (pipettes) should not be dried in an oven. […]

1. Pipettes (pipettes) should not be dried in an oven.
2. Pipettes (pipettes) cannot remove solutions that are too hot or too cold
3. The same pipette should be used as much as possible in the same experiment.
4. After the pipette is raised to the liquid level, wipe off the liquid that sticks to the outer wall of the pipette with filter paper;
5. When looking at the scale, the scale of the pipette should be parallel to the eye, whichever is the lowest meniscus.
6. After using the pipette, it should be washed with tap water and distilled water immediately and placed on the pipette rack.
7. Pipettes and volumetric flasks are often used together, so the relative volume calibration of the two is often done before use.
8. When using the pipette, in order to reduce the measurement error, each time from the top scale (0 scale) as the starting point, the required volume of solution should be dropped down, rather than the volume required to absorb as much volume.
Cleaning of pipette
After washing with tap water, soak with chromic acid detergent. The operation method is as follows:
Hold the pipette or the upper end of the pipette in the right hand. The index finger is close to the upper mouth of the pipette. The middle finger and ring finger open and hold the outside of the pipette. The thumb is held in the middle of the middle finger and ring finger, and the little finger relaxes naturally.Hold the suction ball in the left hand and hold the fist style. Hold the suction ball in the palm with the tip down. Hold the suction ball tightly and expel the air in the ball. Insert or close the tip of the suction ball in the upper mouth of the pipette (suction tube), and pay attention not to air leakage.Slowly loosen the left finger, slowly inhale the detergent into the tube until the part above the scale line, remove the earball, quickly block the upper mouth of the pipette (pipette) with the right index finger, wait for a moment, and put the detergent back into the original bottle.Rinse the inner and outer walls of pipette (pipette) with tap water until no water droplets are hung, then wash with distilled water for 3 times, and control dry water for standby.
Pipette Cleaning TIPS
Pipettes above 1 mL are scrubbed with pipettes, pipettes of 0.1 mL, 0.2 mL and 0.5 mL can be soaked with detergent and cleaned with an ultrasonic cleaner if necessary.As the chromic acid lotion is carcinogenic, it should be avoided as much as possible.If a large number of batches of pipettes are flushed after washing, the flushing bucket can be used. Put the tip of the pipette upward in the bucket, flush with tap water for many times and then rinse with distilled water or ionless water.

TIANTAI BIOLIFE PLASTIC Co., Ltd.